Monoclonal antibody-directed radioimmunoassay of specific cytochromes P-450
A rapid solid phase radioimmunoassay (RIA) for cytochromes P-450 has been developed utilizing specific monoclonal antibodies to major forms of rat liver cytochrome P-450 that are induced by 3-methylcholanthrene (MC-P-450) and phenobarbital (PB-P-450). Monoclonal antibodies (MAbs) that were endogenously labeled with (/sup 35/S)methionine were used to detect MAb-specific cytochromes P-450 in liver microsomes from untreated rats and rats pretreated with 3-methylcholanthrene (MC) or phenobarbital. The competitive binding assays are rapid and can detect cytochrome P-450 in less than 100 ng of microsomal protein. Tthe RIA was used to examine the distribution of MAb-specific cytochromes P-450 in extrahepatic tissues of MC-treated rats; an approximately 30- to 50-fold greater amount of MC-P-450 in liver relative to lung and kidney was observed, which corresponds well with aryl hydrocarbon hydroxylase activity in these tissues. The inducibility of MAb-specific cytochromes P-450 were observed in MC-treated rats, guinea pigs, and C57BL/6 mice, all highly inducible for aryl hydrocarbon hydroxylase; little increase was observed for the relatively noninducible DBA/2 mouse strain.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 6914308
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 259:3
- Country of Publication:
- United States
- Language:
- English
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3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
CYTOCHROMES
RADIOIMMUNOASSAY
MONOCLONAL ANTIBODIES
USES
GUINEA PIGS
METABOLIC ACTIVATION
MICE
PHENOBARBITAL
RATS
SULFUR 35
ANESTHETICS
ANIMALS
ANTIBODIES
ANTICONVULSANTS
AROMATICS
AZINES
BARBITURATES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYPNOTICS AND SEDATIVES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PYRIMIDINES
RADIOASSAY
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)