Immunochemical identification of three proteinic determinants on the archaebacterium Methanococcus vannielii by monoclonal antibodies
Archaebacteria evolved independently from Eubacteria and Eukaryotes and present unique biochemical features. One group, the methanogens, show a variety of cell-walls paralleling their own evolutionary diversity. Chemical analyses revealed proteins in the cell-wall of Methanococcacea but no murein or pseudomurein. Immunochemical studies were begun using a panel of monoclonal antibodies against M. vannielii SB and quantitative micro-immunoenzymatic methods developed for this purpose. Direct binding and inhibition-blocking assays using sugars and aminosugars also indicated absence of these residues in the SB's cell-wall. Three antibodies, however, recognized three different antigenic determinants involving amino acids: Lys, Phe and Thr (antibody 5A); Ser, Try and Tyr (antibody 5B); and Arg, Lys and Phe (antibody 5F). Each site was recognized by one antibody only, and vice versa. These results agree with those provided by well established chemical methods and support the notion that SB possesses a peculiar cell-wall with proteins only. The data also show the resolution power and reliability of monoclonal probes for structural analysis of the bacterial envelope.
- Research Organization:
- NYSDH, Albany
- DOE Contract Number:
- AC02-81ER10880
- OSTI ID:
- 6906026
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 42; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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