rpoD operon promoter used by sigma/sup H/-RNA polymerase in Bacillus subtilis
Three promoters direct transcription of the sigA (rpoD) operon in Bacillus subtilis. Promoters P1 and P2 are used during the exponential growth phase, whereas P3 is used only during the stationary phase. We examined the use of these promoters in promoter-probe plasmids and found that expression from P3 was prevented by a mutation in spoOH, which encodes the secondary RNA polymerase sigma factor sigma/sup H/. Moreover, we found that sigma/sup H/-containing RNA polymerase efficiently and accurately used the P3 promoter in vitro. Evidently, this operon, which is essential for exponential growth, is transcribed during the early phase of sporulation by this secondary form of RNA polymerase. Comparison of the nucleotide sequences of the P3 promoter and the spoVG promoter, which also is used by ..beta../sup H/-RNA polymerase, revealed sequences at the -10 and -35 regions of these promoters that may signal recognition of promoters by sigma/sup H/-RNA polymerase.
- Research Organization:
- Emory Univ. School of Medicine, Atlanta, GA (USA)
- OSTI ID:
- 6906015
- Journal Information:
- J. Bacteriol.; (United States), Vol. 170:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENE REPRESSORS
BIOCHEMICAL REACTION KINETICS
RNA POLYMERASES
GENES
BACILLUS SUBTILIS
GENE OPERONS
PLASMIDS
TRANSCRIPTION
BACILLUS
BACTERIA
CELL CONSTITUENTS
ENZYMES
KINETICS
MICROORGANISMS
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
REACTION KINETICS
TRANSFERASES
550200* - Biochemistry