Structural organization of the porcine and human genes coding for a leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3)
- Institut fuer Humangenetik der Universitaet, Goettingen (Germany)
- INSERM, Marseille (France)
Leydig insulin-like protein (LEY I-L) is a member of the insulin-like hormone superfamily. The LEY I-L gene (designated INSL3) is expressed exclusively in prenatal and postnatal Leydig cells. The authors report here the cloning and nucleotide sequence of porcine and human LEY I-L genes including the 5[prime] regions. Both genes consist of two exons and one intron. The organization of the LEY I-L gene is similar to that of insulin and relaxin. The transcription start site in the porcine and human LEY I-L gene is localized 13 and 14 bp upstream of the translation start site, respectively. Alignment of the 5[prime] flanking regions of both genes reveals that the first 107 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 80%. This conserved region contains a consensus TATAA box, a CAAT-like element (GAAT), and a consensus SP1 sequence (GGGCGG) at equivalent positions in both genes and therefore may play a role in regulation of expression of the LEY I-L gene. The porcine and human genome contains a single copy of the LEY I-L gene. By in situ hybridization, the human gene was assigned to bands p13.2-p12 of the short arm of chromosome 19. 25 refs., 6 figs.
- OSTI ID:
- 6904030
- Journal Information:
- Genomics; (United States), Vol. 20:1; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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GENES
DNA SEQUENCING
DNA-CLONING
HUMAN CHROMOSOME 19
GENETIC MAPPING
PEPTIDE HORMONES
DNA HYBRIDIZATION
TESTES
BODY
CHROMOSOMES
CLONING
GONADS
HORMONES
HUMAN CHROMOSOMES
HYBRIDIZATION
MALE GENITALS
MAPPING
ORGANIC COMPOUNDS
ORGANS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics