The first seven amino acids encoded by the v-src oncogene act as a myristylation signal: Lysine 7 is a critical determinant
The transforming protein of Rous sarcoma virus, pp60/sup v-src/, is covalently coupled to myristic acid by an amide linkage to glycine 2. Myristylation promotes the association of pp60/sup v-src/ with cellular membranes, and this subcellular location is essential for transforming activity. The findings presented here, in conjunction with the previous reports of others, imply that the seventh amino acid encoded by v-src might be important in the myristlyation reaction. Replacement of lysine 7 by asparagine greatly reduced the myristylation, membrane association, and transforming activity of pp60/sup v-src/. In contrast, substitution of arginine at residue 7 had no effect on any of these properties of pp60/sup v-src/. Addition of amino acids 1 to 7 encoded by v-src was sufficient to cause myristylation of a src-pyruvate kinase function protein. The authors conclude that the recognition sequence for myristylation of pp60/sup v-src/ comprises amino acids 1 to 7 and that lysine 7 is a critical component of this sequence.
- Research Organization:
- Departments of Microbiology and Immunology and Biochemistry and Biophysics, and The G.W. Hooper Research Foundation, Univ. of California, San Francisco, CA (US)
- OSTI ID:
- 6903071
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINO ACID SEQUENCE
MOLECULAR BIOLOGY
ONCOGENES
PHOSPHOTRANSFERASES
POST-TRANSLATION MODIFICATION
BIOLOGICAL FUNCTIONS
HYBRIDIZATION
LYSINE
MONOCARBOXYLIC ACIDS
ONCOGENIC VIRUSES
PHOSPHOPROTEINS
PYRUVIC ACID
SUBCELLULAR DISTRIBUTION
AMINO ACIDS
CARBOXYLIC ACIDS
DISTRIBUTION
ENZYMES
FUNCTIONS
GENES
KETO ACIDS
MICROORGANISMS
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
TRANSFERASES
VIRUSES
550200* - Biochemistry