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Title: Hydrocarbon mineralization in sediments and plasmid incidence in sediment bacteria from the Campeche bank

Abstract

Rates of degradation of radiolabeled hydrocarbons and incidence of bacterial plasmid DNA were investigated in sediment samples collected from the Campeche Bank, Gulf of Mexico, site of an offshore oil field containing several petroleum platforms. Overall rates of mineralization of ({sup 14}C) hexadecane and ({sup 14}C)phenanthrene measured for sediments were negligible; <1% of the substrate was converted to CO{sub 2} in all cases. Low mineralization rates are ascribed to nutrient limitations and to lack of adaptation by microbial communities to hydrocarbon contaminants. Plasmid frequency data for sediment bacteria similarly showed no correlation with proximity to the oil field, but, instead, showed correlation with water column depth at each sampling site. Significant differences between sites were observed for proportion of isolates carrying single or multiple plasmids and mean number of plasmids per isolate, each of which increased as a function of depth.

Authors:
; ; ; ; ;  [1]
  1. Univ. of Maryland, College Park (USA)
Publication Date:
OSTI Identifier:
6900051
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (USA)
Additional Journal Information:
Journal Volume: 56:6; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; HEXADECANE; BIODEGRADATION; PHENANTHRENE; BACTERIA; BIOASSAY; CARBON 14 COMPOUNDS; DNA; GULF OF MEXICO; MINERALIZATION; OFFSHORE OPERATIONS; OIL FIELDS; PETROLEUM DEPOSITS; PLASMIDS; SEDIMENTS; TRACER TECHNIQUES; ALKANES; AROMATICS; ATLANTIC OCEAN; CARIBBEAN SEA; CELL CONSTITUENTS; CHEMICAL REACTIONS; CONDENSED AROMATICS; DECOMPOSITION; GEOLOGIC DEPOSITS; HYDROCARBONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; MINERAL RESOURCES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RESOURCES; SEAS; SURFACE WATERS; 020900* - Petroleum- Environmental Aspects

Citation Formats

Leahy, J G, Somerville, C C, Cunningham, K A, Adamantiades, G A, Byrd, J J, and Colwell, R R. Hydrocarbon mineralization in sediments and plasmid incidence in sediment bacteria from the Campeche bank. United States: N. p., 1990. Web.
Leahy, J G, Somerville, C C, Cunningham, K A, Adamantiades, G A, Byrd, J J, & Colwell, R R. Hydrocarbon mineralization in sediments and plasmid incidence in sediment bacteria from the Campeche bank. United States.
Leahy, J G, Somerville, C C, Cunningham, K A, Adamantiades, G A, Byrd, J J, and Colwell, R R. 1990. "Hydrocarbon mineralization in sediments and plasmid incidence in sediment bacteria from the Campeche bank". United States.
@article{osti_6900051,
title = {Hydrocarbon mineralization in sediments and plasmid incidence in sediment bacteria from the Campeche bank},
author = {Leahy, J G and Somerville, C C and Cunningham, K A and Adamantiades, G A and Byrd, J J and Colwell, R R},
abstractNote = {Rates of degradation of radiolabeled hydrocarbons and incidence of bacterial plasmid DNA were investigated in sediment samples collected from the Campeche Bank, Gulf of Mexico, site of an offshore oil field containing several petroleum platforms. Overall rates of mineralization of ({sup 14}C) hexadecane and ({sup 14}C)phenanthrene measured for sediments were negligible; <1% of the substrate was converted to CO{sub 2} in all cases. Low mineralization rates are ascribed to nutrient limitations and to lack of adaptation by microbial communities to hydrocarbon contaminants. Plasmid frequency data for sediment bacteria similarly showed no correlation with proximity to the oil field, but, instead, showed correlation with water column depth at each sampling site. Significant differences between sites were observed for proportion of isolates carrying single or multiple plasmids and mean number of plasmids per isolate, each of which increased as a function of depth.},
doi = {},
url = {https://www.osti.gov/biblio/6900051}, journal = {Applied and Environmental Microbiology; (USA)},
issn = {0099-2240},
number = ,
volume = 56:6,
place = {United States},
year = {Fri Jun 01 00:00:00 EDT 1990},
month = {Fri Jun 01 00:00:00 EDT 1990}
}