Enzymatic method for the synthesis of (/sup 14/C)pyridoxal 5-phosphate from (/sup 14/C)pyridoxine

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90584-1· OSTI ID:6899158

Yagi, T; Takasugi, M; Yamamoto, S; Nozaki, M

A new enzymatic method for the synthesis of (/sup 14/C)pyridoxal 5-phosphate is presented. (/sup 14/C)Pyridoxal 5'-phosphate was synthesized from (/sup 14/C)pyriodoxine through the successive actions of pyridoxal kinase and pyrdoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, (/sup 14/C)pyridoxine, and both enzymes. (/sup 14/C)Pyridoxal 5'-phosphate was isolated by omega-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of (/sup 14/C)pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%.

• Research Organization: Kochi Univ., Nankoku, Japan
• OSTI ID: 6899158
• Journal Information: Anal. Biochem.; (United States), Vol. 158:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
BIOSYNTHESIS
OXIDOREDUCTASES
BIOCHEMICAL REACTION KINETICS
PHOSPHOTRANSFERASES
PYRIDOXAL
ESCHERICHIA COLI
ION EXCHANGE CHROMATOGRAPHY
LIVER
PHOSPHATES
PYRIDOXINE
RATS
THIN-LAYER CHROMATOGRAPHY
ALDEHYDES
ANIMALS
AZINES
BACTERIA
BODY
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PYRIDINES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TRANSFERASES
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques