Acetylcholine receptor-inducing factor from chicken brain increases the level of mRNA encoding the receptor. alpha. subunit

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.6.1983· OSTI ID:6897541

Harris, D A; Falls, D L; Dill-Devor, R M; Fischbach, G D ^[1]

---

1. Washington Univ. School of Medicine, St. Louis, MO (USA)

A 42-kDa glycoprotein isolated from chicken brain, referred to as acetylcholine receptor-inducing activity (ARIA), that stimulates the rate of incorporation of acetylcholine receptors into the surface of chicken myotubes may play a role in the nerve-induced accumulation of receptors at developing neuromuscular synapses. Using nuclease-protection assays, the authors have found that ARIA causes a 2- to 16-fold increase in the level of mRNA encoding the {alpha} subunit of the receptor, with little or no change in the levels of {gamma}- and {delta}-subunit messengers. ARIA also increases the amount of a putative nuclear precursor of {alpha}-subunit mRNA, consistent with an activation of gene transcription. These results suggest that the concentration of {alpha} subunit may limit the rate of biosynthesis of the acetylcholine receptors in chicken myotubes. They also indicate that neuronal factors can regulate the expression of receptor subunit genes in a selective manner. Tetrodotoxin, 8-bromo-cAMP, and forskolin also increase the amount of {alpha}-subunit mRNA, with little change in the amount of {gamma}- and {delta}-subunit mRNAs. Unlike ARIA, however, these agents have little effect on the concentration of the {alpha}-subunit nuclear precursor.

• OSTI ID: 6897541
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOCHEMISTRY
MESSENGER-RNA
AUTORADIOGRAPHY
RECEPTORS
BIOSYNTHESIS
ACETYLCHOLINE
BRAIN
CELL CULTURES
CHICKENS
ENZYME ACTIVITY
HYBRIDIZATION
IODINE 125
MUSCLES
NUCLEASES
PHOSPHORUS 32
RECOMBINANT DNA
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
ESTERS
FOWL
GLYCOPROTEINS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
PROTEINS
QUATERNARY COMPOUNDS
RADIOISOTOPES
RNA
SACCHARIDES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques