Stereotaxic implantation of dispersed cell suspensions into brain. A systematic appraisal of cell placement and survival
The application of several recent advances in cell biology, brain implantation, and cell-mediated tumor immunotherapy requires successful and reproducible placement of viable cell suspensions into brain. Stereotaxic implantation is being used to inject cytotoxic lymphocytes into gliomas and to replace dopaminergic cells in parkinsonian models. Systematic assessment of the factors that influence success in implantation of cell suspensions into solid tissues is needed. A model was developed for investigation of stereotaxic implantation using radiolabeled rat lymphokine-activated killer (LAK) cells. Anesthetized rats received microliter injections of cell suspension into the right caudate nucleus. The injection volume, cell concentration, infusion rate, and needle size were varied systematically. The animals were sacrificed 1 hour after injection; the brain was removed and sectioned, and the radioactivity was counted. Three aliquots of the suspension were injected into counting tubes for control analysis. Recovery of radioactivity was expressed as the percent of mean counts per minute (cpm) in the right frontal lobe/mean cpm in the three control tubes. To assess the viability of implanted cells, the right frontal region was mechanically dissociated in media and centrifuged, and the pellet and supernatant were counted. By using small needles and slow infusion of volumes of 10 microliters or less, 85% to 90% of the radioactivity was recovered in the caudate nucleus. At least half of the implanted cells were viable. Consistent, accurate implantation of dispersed cells into brain over a range of volumes, cell concentrations, infusion rates, and needle sizes was achieved.
- Research Organization:
- National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD (USA)
- OSTI ID:
- 6896794
- Journal Information:
- J. Neurosurg.; (United States), Vol. 69:2
- Country of Publication:
- United States
- Language:
- English
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IMPLANTS
LYMPHOCYTES
SURVIVAL CURVES
BIOLOGICAL MODELS
CELL CULTURES
LYMPHOKINES
RADIOISOTOPES
RATS
SUSPENSIONS
TRACER TECHNIQUES
VIABILITY
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CENTRAL NERVOUS SYSTEM
CONNECTIVE TISSUE CELLS
DISPERSIONS
GROWTH FACTORS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
550901* - Pathology- Tracer Techniques