Longitudinal study of the in vivo hprt mutant frequency in human T-lymphocytes as determined by a cell cloning assay
- Univ. of Vermont, Burlington (USA)
The in vivo frequency of mutants resulting from mutation at the hprt locus in human T-lymphocytes can be determined by a cloning assay. This assay quantifies the frequency of 6-thioguanine-resistant (TG{sup r}) T-cells through growth of colonies in 96-well microtiter dishes. The reproducibility of the TG{sup r} mutant frequency values has now been assessed in a longitudinal study of six individuals employing 4-5 blood samples over a 26-37 week time period. Cloning assays were performed with both fresh and cryopreserved cell samples. No significant differences were found among the mutant frequency values for multiple samples from each individual with both fresh and cryopreserved cell samples. These results demonstrate the reproducibility of this cloning assay for in vivo mutant frequency determinations in human T-lymphocytes.
- OSTI ID:
- 6896781
- Journal Information:
- Environmental and Molecular Mutagenesis; (USA), Vol. 13:4; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MUTATION FREQUENCY
LYMPHOCYTES
CYTOLOGICAL TECHNIQUES
CLONE CELLS
GUANINE
MAN
RELIABILITY
THIOLS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
ENZYMES
GLYCOSYL TRANSFERASES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PENTOSYL TRANSFERASES
PRIMATES
PURINES
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
550400* - Genetics