Angiotensin II receptors in rabbit renal preglomerular vessels

Journal Article · Fri Jul 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6896351

Brown, G P; Venuto, R C ^[1]

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1. State Univ. of New York, Buffalo (USA)

Controversy exists regarding the specific sites within the renal microcirculation affected by angiotensin II (ANG II). Under some conditions, ANG II can elicit direct vasoconstrictor responses in the preglomerular vessels and efferent arterioles. These experiments were designed to evaluate the binding of {sup 125}I-ANG II in preglomerular vessels. Arcuate and interlobular arteries, with attached proximal segments of afferent arterioles, were microdissected from rabbit renal cortexes. A membrane preparation was obtained from the pooled freshly dissected vessels and utilized in an ANG II radioreceptor assay on the same day. The dissociation of bound ANG II was enhanced in the presence of a nonhydrolyzable analogue of GTP. Linear Scatchard plots were obtained, indicating the presence of a single class of high-affinity binding sites. In conclusion, there is a single class of specific ANG II receptors in preglomerular vessels. The K{sub D} and N are similar, but the binding inhibition potencies of selected ANG analogues differ in renal and extrarenal vascular tissues. Intrarenal vascular receptors also appear to differ from glomerular receptors. Furthermore, these data support the concept that ANG II may affect renal vascular resistance at sites proximal to the distal afferent arterioles.

• OSTI ID: 6896351
• Journal Information: American Journal of Physiology; (USA), Vol. 255:1; ISSN 0002-9513
• Country of Publication: United States
• Language: English

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DOSE-RESPONSE RELATIONSHIPS
IODINATION
IODINE 125
KIDNEYS
NUCLEOTIDES
PREGNANCY
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ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
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