Correlated analysis of cellular DNA, membrane antigens and light scatter of human lymphoid cells
Flow cytometric correlated analysis of membrane antigens, DNA, and light scatter was performed on human lymphoid cells using fluorescein (FITC)-conjugated antibodies to label B- and T-cell antigens and propidium iodide (PI) to stain DNA after ethanol fixation and RNase treatment. A FACS II flow cytometer was modified to obtain digitized measurements of two color fluorescence and light scatter emissions, simultaneously. Software was written to allow single parameter analysis or correlated analysis of any two of the three parameters acquired. Ethanol fixation preserved FITC surface labeling for at least 15 weeks, but produced marked changes in light scatter. No changes in FITC distributions were observed after RNase treatment and PI staining, and the presence of FITC labeling did not affect DNA distributions. Within heterogeneous cell populations, the DNA distribution of cell subpopulations identified by a membrane antigen was clearly demonstrated.
- Research Organization:
- Department of Pathology, University of Florida College of Medicine, Gainesville
- OSTI ID:
- 6880110
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 2:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
BIOCHEMISTRY
MORPHOLOGY
ANTIGENS
CELL FLOW SYSTEMS
DNA
LIGHT SCATTERING
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SCATTERING
SOMATIC CELLS
550300* - Cytology