Urease testing of mycobacteria with BACTEC radiometric instrumentation

Journal Article · Mon Mar 01 00:00:00 EST 1982 · J. Clin. Microbiol.; (United States)

OSTI ID:6879656

Damato, J J; Collins, M T; McClatchy, J K

A total of 140 mycobacterial isolates from patients treated at Fitzsimons Army Medical Center or the National Jewish Hospital and Research Center and from animal specimens submitted to the National Veterinary Services Laboratory were tested by using a urease procedure modified for use with a BACTEC model 301. Mycobacterial suspensions were prepared by using Middlebrook 7H10 Tween broth. Of the 98 mycobacteria isolates which were urease positive utilizing standard methodology, all were positive using the radiometric procedures. Similarly, all 42 urease-negative isolates were also negative employing the new methodology. Although maximum radiometric readings were observed at 48 h, all positive strains were readily identified 24 h after inoculation without sacrificing either test sensitivity or specificity. Thus, urease testing of mycobacteria, using the modified BACTEC radiometric methodology, was as sensitive, as specific, and more rapid than conventional methods.

• Research Organization: Clinical Investigation Service, Fitzsimons Army Medical Center, Aurora, Colorado
• OSTI ID: 6879656
• Journal Information: J. Clin. Microbiol.; (United States), Vol. 15:3
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
BACTERIAL DISEASES
DIAGNOSIS
CARBON ISOTOPES
ISOTOPE APPLICATIONS
MYCOBACTERIUM
RADIOMETRIC ANALYSIS
PATIENTS
UREASE
AMIDINASES
BACTERIA
CHEMICAL ANALYSIS
DISEASES
ENZYMES
HYDROLASES
INFECTIOUS DISEASES
ISOTOPES
MICROORGANISMS
NON-PEPTIDE C-N HYDROLASES
QUANTITATIVE CHEMICAL ANALYSIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550701 - Microbiology- Tracer Techniques