Characterization of biophysical properties of baboon lipoproteins: modulation by dietary fat and cholesterol
The serum lipoproteins of baboons fed diets containing differing types and amounts of fat and varying amounts of cholesterol were examined by analytic ultracentrifugation, gradient gel electrophoresis, density gradient ultracentrifugation, sodium dodecyl sulfate-polyacrylamide electrophoresis, electron microscopy, and standard protein and lipid composition assays. These studies characterized the lipoproteins of the baboon, observed how concentrations and physical-chemical properties of the lipoproteins are modulated by dietary fat and cholesterol and described the suitability of the baboon as an animal model of human lipoprotein metabolism. Results indicate that baboon high density lipoproteins (HDL), though higher in total serum concentration than human HDL, are remarkably similar to human HDL. The concentration of baboon HDL is increased by dietary saturated fat but decreased by the addition of cholesterol. While serum concentrations of low density lipoproteins (LDL) tend to be lower in baboons, the physical-chemical properties of the LDL of baboons and humans are comparable. The LDL of both species contains apolipoprotein B as their major apolipoprotein and exhibit considerable polydispersity in particle size. LDL of both species consists of seven discrete subpopulations. The analytical and statistical data presented in this dissertation indicate that the baboon is a good model for studying the role of lipoproteins in the development of atherosclerosis. 125 references, 31 figures, 28 tables.
- Research Organization:
- Lawrence Berkeley Lab., CA (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6879305
- Report Number(s):
- LBL-17826; ON: DE84013401
- Resource Relation:
- Other Information: Portions are illegible in microfiche products. Thesis
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARTERIOSCLEROSIS
BIOLOGICAL MODELS
LIPOPROTEINS
BLOOD CHEMISTRY
QUANTITATIVE CHEMICAL ANALYSIS
APES
CENTRIFUGATION
CHOLESTEROL
DIET
ELECTRON MICROSCOPY
ELECTROPHORESIS
EXPERIMENTAL DATA
FATS
PHOSPHOLIPIDS
TRIGLYCERIDES
ANIMALS
CARDIOVASCULAR DISEASES
CHEMICAL ANALYSIS
DATA
DISEASES
ESTERS
HYDROXY COMPOUNDS
INFORMATION
LIPIDS
MAMMALS
MICROSCOPY
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PRIMATES
PROTEINS
SEPARATION PROCESSES
STEROIDS
STEROLS
VASCULAR DISEASES
VERTEBRATES
550900* - Pathology
550200 - Biochemistry