Induction of chromosome damage by ultraviolet light and caffeine: correlation of cytogenetic evaluation and flow karyotype
Asynchronously growing cells of a M3-1 Chinese hamster line were ultraviolet (UV) irradiated (lambda . 254 nm) with UV fluences up to 7.5 J/m(2). After irradiation cells were incubated with or without 2 mM caffeine for 20 hr, then mitotic cells were selected by mechanical shaking. Their chromosomes were isolated, stained with Hoechst 33258 and chromomycin A3, and measured flow cytometrically. While the fluorescence distributions of chromosomes (flow karyo-types) from cells treated with UV alone or with caffeine alone were very similar to those of untreated controls, the flow karyo-types of UV + caffeine-treated cells showed a debris continuum that increased with increasing UV fluence suggesting an increased number of chromosome fragments. Visual evaluation of metaphase plates revealed that the percentage of cells with chromosome damage also increased steadily with increasing UV fluence. A high degree of correlation was observed between the relative magnitude of the debris level from flow karyotypes and the percentage of cells with chromosome damage and with generalized chromosomes shattering, respectively, as determined from metaphase spreads.
- Research Organization:
- Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA
- OSTI ID:
- 6878992
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 2:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Replication kinetics of Chinese hamster chromosomes as revealed by bivariate flow karyotyping
Generation of five high-complexity painting probe libraries from flow-sorted mouse chromosomes
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CAFFEINE
RADIOSENSITIVITY EFFECTS
CHROMOSOMES
BIOLOGICAL RADIATION EFFECTS
ANIMAL CELLS
CELL CULTURES
CELL FLOW SYSTEMS
LABORATORY ANIMALS
MITOSIS
SENSITIVITY
ULTRAVIOLET RADIATION
ANALEPTICS
BIOLOGICAL EFFECTS
CELL DIVISION
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
XANTHINES
560121* - Radiation Effects on Cells- External Source- (-1987)
550301 - Cytology- Tracer Techniques