Elongating internodes of Zea mays (maize): Early steps in the GA biosynthetic pathway

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6873595

Suzuki, Y; Phinney, B O ^[1]; Gaskin, P; MacMillan, J ^[2]

---

1. Univ. of California, Los Angeles (USA)
2. Univ. of Bristol (England)

The early steps in the gibberellin (GA) biosynthetic pathway have yet to be defined for tissues that show a growth response to GAs. To this end we have synthesized the ({sup 13}C,{sup 3}H)-ent-kaurenoids, ent-kaurenol, ent-kaurenal ent-kaukenoic acid. We also have double-labeled ent-kaurene and double-labeled GA{sub 12}-aldehyde. We feed 1 - 10{mu}g of each substrate, individually, to 1.0g diced internodes in the appropriate buffer plus cofactors. We have observed up to 80% metabolism. We have identified (full scan GC-MS) 7{beta}-hydroxy-ent-kaurenoic acid as the major metabolite from double-labeled ent-kaurenoic acid feeds, thus defining the step ent-kaurenoic acid to 7{beta}-hydroxy-ent-kaurenoic acid.

• OSTI ID: 6873595
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GIBBERELLIC ACID
METABOLISM
MAIZE
PLANT GROWTH
BIOLOGICAL PATHWAYS
CARBON 13
DOUBLE LABELLING
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CARBON ISOTOPES
CARBOXYLIC ACIDS
CEREALS
CHROMATOGRAPHY
ESTERS
EVEN-ODD NUCLEI
GRASS
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LACTONES
LIGHT NUCLEI
LILIOPSIDA
MAGNOLIOPHYTA
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
550501* - Metabolism- Tracer Techniques