HincII and KpnI RFLPs for laminin B1 (LAMB1) gene on chromosome 7

Journal Article · Mon Sep 12 00:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.17.8742· OSTI ID:6871781

Ardinger, H H; Rose, K M; Murray, J C ^[1]; Yamada, Y ^[2]

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1. Univ. of Iowa, Iowa City (USA)
2. National Institute of Dental Research, Bethesda, MD (USA)

p1236 was isolated from a human endothelial cell cDNA library in {lambda}gt11. It is a 1.2 kb insert subcloned into the EcoRI site of pUC19. HincII identifies a two allele polymorphism with bands of 7 or 5.8 and 1.2 kb. KpnI identifies a two allele polymorphism with bands of 40 or 25 and 15 kb. The probe was localized to 7q31 using in situ hybridization. Co-dominant segregation was shown in 3 (hincII) and 7(KpnI) families. A rare polymorphism with BamHI was observed in one person. Linkage disequilibrium appears to be present between HincII and KpnI alleles. A double digest of SacI with KpnI allows better visualization of the KpnI bands.

• OSTI ID: 6871781
• Journal Information: Nucleic Acids Research; (UK), Vol. 16:17; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RFLPS
GENETIC MAPPING
DNA HYBRIDIZATION
DNA SEQUENCING
ENDOTHELIUM
HUMAN POPULATIONS
MAN
PROTEINS
RECOMBINANT DNA
ANIMAL TISSUES
ANIMALS
BODY
DNA
HYBRIDIZATION
MAMMALS
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POPULATIONS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
TISSUES
VERTEBRATES
550200* - Biochemistry