Interleukin 3 perfusion in W/Wv mice allows the development of macroscopic hematopoietic spleen colonies and restores cutaneous mast cell number

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Journal of Experimental Medicine; (USA)

DOI:https://doi.org/10.1084/jem.172.1.403· OSTI ID:6868948

Ody, C; Kindler, V; Vassalli, P ^[1]

---

1. Univ. of Geneva (Switzerland)

The genetically anemic W/Wv mice are characterized by the inability of their bone marrow cells to form macroscopic pluripotent hematopoietic colonies in the spleen of irradiated recipients upon transfer (colony-forming units). Furthermore, they almost totally lack mast cells, notably in the skin. In the present study, we have tested the effect of recombinant murine interleukin 3 (rmIL-3) on W/Wv mice hematopoiesis. Transfer of W/Wv bone marrow cells into lethally irradiated recipients perfused with rmIL-3 is followed by the appearance of macroscopic spleen colonies. Moreover, perfusion of rmIL-3 in W/Wv mice: (a) restores almost normal total numbers of hematopoietic precursors (colony-forming cells), but without modification of anemia; and (b) leads to the appearance of a normal number of mastocytes in the skin.

• OSTI ID: 6868948
• Journal Information: Journal of Experimental Medicine; (USA), Vol. 172:1; ISSN 0022-1007
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
TRANSPLANTS
LYMPHOKINES
BIOLOGICAL FUNCTIONS
MAST CELLS
COLONY FORMATION
ANEMIAS
CELL CULTURES
HEMATOPOIETIC SYSTEM
LETHAL IRRADIATION
MICE
PRECURSOR
PROTEINS
SPLEEN
ANIMAL CELLS
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
DISEASES
FUNCTIONS
GROWTH FACTORS
HEMIC DISEASES
IRRADIATION
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals