Dermal penetration and systemic distribution of sup 14 C-labeled vitamin E human skin grafted athymic nude mice
In vivo percutaneous penetration and tissue distribution of 14C-labeled vitamin E applied to human skin grafted onto athymic nude mice were determined. At 1 hr, mouse skin contained the highest level of radioactivity, followed by the muscle, blood, liver, lung, adipose tissue, spleen, kidney, brain, heart, and eyes. A linear increase with time in tissue radioactivity was observed throughout the 24 hr experimental period. At 4 and 24 hrs skin grafts were highly radioactive. At 4 hrs the epidermis and the upper portion of the dermis contained more radioactivity than the remaining portion of the dermis. In contrast, at 24 hrs the highest level of radioactivity was detected in the lower dermis. No radioactivity was detected in expired air while 0.2% of the dose was found in the urine. The data show that vitamin E does penetrate skin and that the dermis acts as a barrier or reservoir for this highly lipophilic compound.
- Research Organization:
- Letterman Army Inst. of Research, San Francisco, CA (USA)
- OSTI ID:
- 6867986
- Report Number(s):
- AD-A-222838/5/XAB
- Resource Relation:
- Other Information: Pub. in International Jnl. for Vitamin and Nitrition Research, Vol. 59, 333-338(13 Mar 1989)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SKIN
GRAFTS
VITAMIN E
TISSUE DISTRIBUTION
ADIPOSE TISSUE
CARBON 14 COMPOUNDS
METABOLISM
MICE
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BODY
CONNECTIVE TISSUE
DISTRIBUTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANS
RODENTS
TISSUES
TRANSPLANTS
VERTEBRATES
VITAMINS
550501* - Metabolism- Tracer Techniques