Pleiotropic mutations at the TUP1 locus that affect the expression of mating-type-dependent functions in Saccharomyces cerevisiae
- Oak Ridge National Lab., TN
The umr7-1 mutation, previously identified in a set of mutants that had been selected for defective uv-induced mutagenesis at CAN1, affects other cellular functions, including many of those regulated by the mating-type locus (MAT) in heterothallic Saccharomyces cerevisiae. The recessive umr7-1 allele, mapping approximately 20 cM distal to thr4 on chromosome III, causes clumpy growth in both a and ..cap alpha.. cells and has no apparent effect on a mating functions. The most striking property of a umr7 strains is their altered morphology, in which mitotic cells develop an asymmetric pear shape, like that of normal a cells induced to form shmoos by interaction with a-factor. Some a/..cap alpha..-specific diploid functions are also affected by umr7; instead of polar budding patterns, a/..cap alpha.. umr7/umr7 diploids have medial budding like a/a, ..cap alpha../..cap alpha.. and haploid strains. Moreover, a/..cap alpha.. umr7/umr7 diploids have lost the ability to sporulate and are Bar+ like a or a/a strains. Revertant studies indicate that umr7-1 is a single point mutation. The umr7 mutant fails to complement mutants of both tup1 and cyc9 and all three isolates have similar genetic and phenotypic properties. It is suggested that the product of this gene plays some common central role in the complex regulation of the expression of both MAT-dependent and MAT-independent functions.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6866548
- Journal Information:
- Genetics; (United States), Vol. 94:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
GENE MUTATIONS
RADIOINDUCTION
MORPHOLOGY
GENETIC VARIABILITY
SACCHAROMYCES CEREVISIAE
GENETIC RADIATION EFFECTS
GENETIC MAPPING
REVERTANTS
ULTRAVIOLET RADIATION
ZYGOTES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
ELECTROMAGNETIC RADIATION
FUNGI
GENETIC EFFECTS
MAPPING
MICROORGANISMS
MUTANTS
MUTATIONS
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
YEASTS
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