Evaluation of (/sup 3/H)thymidine uptake method for studying growth of spiroplasmas under various conditions

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · J. Clin. Microbiol.; (United States)

OSTI ID:6866311

Bastian, F O; Baliga, B S; Pollock, H M

(/sup 3/H)thymidine uptake and colony counts are quantitative and inexpensive methods for studying Spiroplasma growth. Using these techniques, we demonstrated subtle effects on the growth of suckling mouse cataract agent of medium alterations, inoculum size, and freezing of cultures. In addition, suckling mouse cataract agent multiplied more actively under aerobic than under anaerobic conditions. These techniques have wide application for the study of Spiroplasma growth and will be useful for the development of a defined medium.

• Research Organization: Univ. of South Alabama, Mobile (USA)
• OSTI ID: 6866311
• Journal Information: J. Clin. Microbiol.; (United States), Vol. 26:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
GROWTH
THYMIDINE
UPTAKE
CULTURE MEDIA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AZINES
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
550701* - Microbiology- Tracer Techniques