Imaging of cardiac allograft rejection in dogs using indium-111 monoclonal antimyosin Fab

Journal Article · Sun Mar 01 00:00:00 EST 1987 · J. Am. Coll. Cardiol.; (United States)

DOI:https://doi.org/10.1016/S0735-1097(87)80048-7· OSTI ID:6862991

Addonizio, L J; Michler, R E; Marboe, C; Esser, P E; Johnson, L L; Seldin, D W; Gersony, W M; Alderson, P O; Rose, E A; Cannon, P J

The acute rejection of cardiac allografts is currently diagnosed by the presence of myocyte necrosis on endomyocardial biopsy. We evaluated the efficacy of noninvasive scintigraphic imaging with indium-111-labeled anticardiac myosin Fab fragments (indium-111 antimyosin) to detect and quantify cardiac allograft rejection. Six dogs that had intrathoracic heterotopic cardiac allograft transplantation were injected with indium-111 antimyosin and planar and single photon emission computed tomographic (SPECT) images were obtained in various stages of acute and subacute rejection. Four dogs had an allograft older than 8 months and had been on long-term immunosuppressive therapy; two dogs had an allograft less than 2 weeks old and were not on immunosuppressive therapy. Count ratios comparing heterotopic with native hearts were calculated from both SPECT images and in vitro scans of excised and sectioned hearts and were compared with the degree of rejection scored by an independent histopathologic review. Indium-111 antimyosin uptake was not visible in planar or SPECT images of native hearts. Faint diffuse uptake was apparent in cardiac allografts during long-term immunosuppression and intense radioactivity was present in hearts with electrocardiographic evidence of rejection. The heterotopic to native heart count ratios in SPECT images correlated significantly with the count ratios in the excised hearts (r = 0.93) and with the histopathologic rejection score (r = 0.97). The distribution of indium-111 antimyosin activity in right and left ventricles corresponded to areas of histopathologic abnormalities.

• Research Organization: Columbia Univ., New York, NY (United States)
• OSTI ID: 6862991
• Journal Information: J. Am. Coll. Cardiol.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
GRAFT-HOST REACTION
DIAGNOSIS
HEART
SCINTISCANNING
TRANSPLANTS
MONOCLONAL ANTIBODIES
DIAGNOSTIC USES
DOGS
ELECTROCARDIOGRAMS
IMMUNE REACTIONS
INDIUM 111
MYOCARDIUM
MYOSIN
NECROSIS
SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
COMPUTERIZED TOMOGRAPHY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIAGRAMS
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
GLOBULINS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
TOMOGRAPHY
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics