Development of neural crest cells expressing nerve growth factor receptors
The present study examines the ontogeny of the nerve growth factor receptor of neural crest cells in vitro and the phenotypic nature of the neural crest cells expressing this receptor. /sup 125/I-NGF binding assays and autoradiographic and immunofluorescence techniques have demonstrated the presence of a subpopulation of quail neural crest cells that express specific NGF receptors after 3-4 days in vitro. This subpopulations represents approximately 28% of the cells in 5-day primary cultures and 30-35% of the cells in secondary cultures; these cells generally exhibited a flattened, phase-dark morphology. Approximately one-third of these cells also labeled with a 2 hr pulse of /sup 3/H thymidine. Catecholamine-containing neural crest cells generally lacked NGF receptors. NGF receptor-positive cells also failed to demonstrate somatostatin-, neuron-specific enolase-, or S-100-like immunoreactivity. Melanocytes do not appear to express NGF receptors. Exogenous nerve growth factor did not influence the morphology or mitotic status of the cells in culture.
- Research Organization:
- Chicago Univ., IL (USA)
- OSTI ID:
- 6861954
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expression of the capacity to release (/sup 3/H)norepinephrine by neural crest cultures
Synthesis on accumulation of putative neurotransmitters by cultured neural crest cells