Antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of UV B-irradiated mice
UV B-irradiation (280 to 320 nm) of mice at the site of cutaneous infection with herpes simplex virus type 2 (HSV-2) induced suppressor T-cell circuits that decreased HSV-2-induced proliferative responses of HSV-2-immune lymph node cells. Adoptive transfer experiments indicated that splenocytes from UV B-irradiated HSV-2-infected animals contain L3T4+ cells that suppress proliferative responses in vivo, consistent with suppressor inducer cells. However, following in vitro culture of the splenocytes with HSV-2 antigen, the proliferation of immune lymph node cells was inhibited by Lyt2+ suppressor T cells, consistent with antigen-induced suppressor effector cells. Antigen-specific and nonspecific suppressor factors were fractionated from supernatants of HSV-2-stimulated spleen cells by molecular-sieve chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the Sephadex fraction that contained the antigen-specific suppressor factor, in the presence or absence of 2-mercaptoethanol, defined a 115-kilodalton protein consisting of two disulfide-bound components with molecular sizes of 70 and 52 kilodaltons. The implications of these results with respect to the regulation of HSV-induced cell-mediated immunity following UV B-irradiation are discussed.
- Research Organization:
- Univ. of Maryland School of Medicine, Baltimore (USA)
- OSTI ID:
- 6856410
- Journal Information:
- J. Virol.; (United States), Vol. 62:7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
IMMUNITY
RADIOINDUCTION
LYMPHOCYTES
CELL PROLIFERATION
CHROMATOGRAPHY
ELECTROPHORESIS
HERPES SIMPLEX
INHIBITION
LYMPH NODES
MICE
SPLEEN CELLS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
INFECTIOUS DISEASES
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
RADIATIONS
RODENTS
SEPARATION PROCESSES
SKIN DISEASES
SOMATIC CELLS
VERTEBRATES
VIRAL DISEASES
560152* - Radiation Effects on Animals- Animals