Pharmacokinetic analysis of the plasma disappearance of ovine follitropin and analogues in the male rat

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90094-8· OSTI ID:6855005

Sebok, K; Meloche, S; Sairam, M R ^[1]

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1. Clinical Research Institute of Montreal, Quebec (Canada)

Ovine follitropin (oFSH) and its chemically deglycosylated (DG) or the asialo analogue were injected as a single bolus to intact mature male rats and plasma levels measured by radioimmunoassay. Semi-logarithmic plots of the plasma disappearance curves were all biphasic. For all substances tested, the data were best fitted to a biexponential equation and the following parameters were calculated: the half-lives of the distribution and elimination phases and the metabolic clearance rate (MCR) at steady state. It was found that the distribution phase of oFSH was much slower than for deglycosylated (DG) {alpha} + {beta} recombinant, DG-oFSH and asialo-oFSH. The elimination half-lives for asialo-oFSH and DG {alpha} + {beta} recombinant were much shorter than for oFSH and DG-oFSH. oFSH had the lowest MCR, followed by DG {alpha} + {beta}, DG-oFSH and asialo-oFSH. This study clearly shows that sugar residues internal to sialic acid also play a significant part in the clearance of the hormone.

• OSTI ID: 6855005
• Journal Information: Life Sciences; (USA), Vol. 46:13; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FSH
METABOLISM
BIOLOGICAL HALF-LIFE
RADIOIMMUNOASSAY
RATS
STEADY-STATE CONDITIONS
ANIMALS
BIOASSAY
DIAGNOSTIC TECHNIQUES
GONADOTROPINS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550501* - Metabolism- Tracer Techniques