Potentially lethal and DNA radiation damage: similarities in inhibition of repair by medium containing D/sub 2/O and by hypertonic buffer
Killing of log-phase V79 Chinese hamster cells by ionizing radiation is enhanced when cells are treated immediately after irradiation with anisotonic phosphate-buffered saline or with medium containing 90% D/sub 2/O. These treatments are not toxic to unirradiated cells. In medium containing D/sub 2/O, cells are unable to repair sublethal damage; however, repair occurs after a shift to normal medium. Thus, as is observed with anisotonic buffer, enhanced killing by medium containing D/sub 2/O does not prevent cells from repairing sublethal damage. Further aspects of the enhanced expression of potentially lethal damage by medium containing D/sub 2/O and by anisotonic buffer are also similar. For example, both are largely ineffective after treatments involving nonionizing radiation, and both treatments retard the repair of DNA breaks. From the foregoing, and the partial overlapping of enhanced killing due to medium containing D/sub 2/O and due to hypertonic saline, we infer that the latter treatments affect the repair, or reparability, of the same sensitive targets.
- Research Organization:
- Argonne National Lab., IL
- DOE Contract Number:
- W-31-109-ENG-38
- OSTI ID:
- 6853795
- Journal Information:
- Radiat. Res.; (United States), Vol. 84:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOLOGICAL REPAIR
INHIBITION
DNA
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
BUFFERS
CELL CULTURES
CELL KILLING
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
HAMSTERS
HEAVY WATER
IONIZING RADIATIONS
LETHAL DOSES
STRAND BREAKS
SURVIVAL CURVES
TIME DEPENDENCE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
DATA
DOSES
HYDROGEN COMPOUNDS
INFORMATION
MAMMALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
VERTEBRATES
WATER
560121* - Radiation Effects on Cells- External Source- (-1987)