Chromosome I duplications in Caenorhabditis elegans
- Univ. of British Columbia, Vancouver (Canada)
We have isolated and characterized 76 duplications of chromosome I in the genome of Caenorhabditis elegans. The region studied is the 20 map unit left half of the chromosome. Sixty-two duplications were induced with gamma radiation and 14 arose spontaneously. The latter class was apparently the result of spontaneous breaks within the parental duplication. The majority of duplications behave as if they are free. Three duplications are attached to identifiable sequences from other chromosomes. The duplication breakpoints have been mapped by complementation analysis relative to genes on chromosome I. Nineteen duplication breakpoints and seven deficiency breakpoints divide the left half of the chromosome into 24 regions. We have studied the relationship between duplication size and segregational stability. While size is an important determinant of mitotic stability, it is not the only one. We observed clear exceptions to a size-stability correlation. In addition to size, duplication stability may be influenced by specific sequences or chromosome structure. The majority of the duplications were stable enough to be powerful tools for gene mapping. Therefore the duplications described here will be useful in the genetic characterization of chromosome I and the techniques we have developed can be adapted to other regions of the genome.
- OSTI ID:
- 6853401
- Journal Information:
- Genetics; (USA), Vol. 124:1; ISSN 0016-6731
- Country of Publication:
- United States
- Language:
- English
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GENE MUTATIONS
RADIOINDUCTION
NEMATODES
GENETIC MAPPING
CHROMOSOMES
GAMMA RADIATION
GENE RECOMBINATION
GENES
GENOTYPE
MITOSIS
SPONTANEOUS MUTATIONS
ASCHELMINTHES
CELL DIVISION
ELECTROMAGNETIC RADIATION
HELMINTHS
IONIZING RADIATIONS
MAPPING
MUTATIONS
RADIATIONS
560152* - Radiation Effects on Animals- Animals