On the sensitivity of intact cells to perturbation by ethanol
- Department of Psychiatry and Behavioral Sciences, Stony Brook, NY (USA)
- Psychiatry Service, Northport, NY (USA)
A comparison was made of ethanol's effects on the order of plasma membranes in intact cells and some isolated membrane preparations. Order was assessed by steady-state fluorescence polarization techniques using the non-permeant probe, TMA-DPH. The data show that two cultured cells, rat neonatal astroglial and N2A neuroblastoma, were sensitive to significant ethanol-induced disordering within the anesthetically relevant range. Human erythrocytes, cultured fibroblasts and homogenized astroglial cells required higher ethanol concentrations to produce a similar effect. Intact erythrocytes were approximately twice as sensitive as erythrocyte ghost membranes to ethanol induced perturbation. The neonatal glial and N2A cells were approximately five times more sensitive than synaptic membranes to ethanol effects. DMPC and DMPC + cholesterol liposomes and myelin membranes were insensitive to ethanol's effects. The incorporation of 10 mole % ganglioside GM{sub 1} sensitized the liposomes to ethanol-induced perturbation.
- OSTI ID:
- 6853096
- Journal Information:
- Life Sciences; (USA), Vol. 45:26; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
SENSITIVITY
ETHANOL
BIOLOGICAL EFFECTS
ANIMAL CELLS
COMPARATIVE EVALUATIONS
ERYTHROCYTES
FIBROBLASTS
FLUORESCENCE
LIPOSOMES
MAN
RATS
STEADY-STATE CONDITIONS
ALCOHOLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
HYDROXY COMPOUNDS
LUMINESCENCE
MAMMALS
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANOIDS
PRIMATES
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology