Dietary essential fatty acids, prostaglandins (PG) production and cellular cytotoxic activity in Balb/c mice
Several investigators have implicated PG as natural feedback inhibitors of cellular cytotoxic activity. It was of interest to determine if dietarily modulated PG synthesis alters cytotoxic response to a varial challenge. Weanling Balb/c mice were fed ad libitum semi-purified diets with 10% by weight corn oil or linseed oil, providing a 1:42 or 2:1 ratio of 18:3n-3 to 18:2n-6, respectively for 4-5 weeks. Mice were then immunochallenged with an intraperitoneal injection of 10/sup 4/ or 10/sup 5/ vaccinia virus. Peritoneal exudate cells and splenocytes were collected on days 3,6 and 9 post-challenge and assessed for cytotoxic activities using the /sup 51/Cr release assay. Peritoneal macrophage (M0), splenic natural killer and cytotoxic T lymphocyte cytotoxic activities were tested against the SV3T3, YAC-1, and vaccinia virus-infected 3T3 cell lines, respectively. PGE/sub 2/ and PGF/sub 2..cap alpha../ production by M0 and splenocytes from dietarily treated, non-challenged mice was determined by radioimmunoassay of cell supernatants following a 60 minute exposure to the calcium ionophore A23187. Peritoneal M0 and splenocyte populations from the linseed oil fed mice produced approximately 50% of the PGE/sub 2/ and PGF/sub 2..cap alpha../ as compared to the corn oil fed mice. However, no significant differences in cellular cytotoxic activity between the linseed oil and corn oil fed mice were observed.
- Research Organization:
- Univ. of Illinois, Urbana
- OSTI ID:
- 6851323
- Report Number(s):
- CONF-8604222-; TRN: 87-005737
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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