Glucose transport by radiation-induced insulinoma and clonal pancreatic beta-cells
Sugar uptake was measured in dispersed cells prepared from radiation-induced insulinomas transplantable in NEDH rats and in three clonal beta-cell lines maintained in continuous culture (RIN m5F, RIN 1046, HIT). Uptake of D-glucose and 3-O-methyl-D-glucose by insulinoma cells was rapid so that the intracellular concentration of D-hexoses approximated the concentration in the incubation medium by 15-30 s. L-Glucose was taken up only slowly. 3-O-methyl-D-glucose uptake by RIN m5F, RIN 1046, and HIT cells was slow; with 1 mM 3-O-methylglucose in the medium, equilibrium was attained at 20 min, but with 10 mM 3-O-methylglucose, equilibrium was not attained even at 20 min. In HIT cells incubated with D-glucose for 30 min, the intracellular concentration of glucose was less than the medium glucose concentration, indicating glucose transport is a nonequilibrium reaction in this cell line. These data indicate that radiation-induced insulinoma cells retain the capacity of normal beta-cells to transport sugar at high rates. RIN m5F, RIN 1046, and HIT cells transport sugar slowly, however, and thus differ from normal beta-cells. In RIN m5F, RIN 1046, and HIT cells, unlike in normal beta-cells, glucose transport may be the site regulating glucose metabolism.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 6847485
- Journal Information:
- Diabetes; (United States), Vol. 12
- Country of Publication:
- United States
- Language:
- English
Similar Records
Stimulatory effects of maitotoxin on insulin release in insulinoma HIT cells: Role of calcium uptake and phosphoinositide breakdown
Evidence that downregulation of hexose transport limits intracellular glucose in 3T3-L1 fibroblasts
Related Subjects
GLUCOSE
UPTAKE
SOMATIC CELLS
BIOLOGICAL RADIATION EFFECTS
CLONE CELLS
INSULIN
PANCREAS
RATS
STRUCTURE-ACTIVITY RELATIONSHIPS
ALDEHYDES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CELL CULTURES
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
GLANDS
HEXOSES
HORMONES
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RADIATION EFFECTS
RODENTS
SACCHARIDES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture