Lipoprotein receptors in copper-deficient rats: high density lipoprotein binding to liver membranes
In copper-deficient rats, the observed hyperlipoproteinemia was mainly due to the elevation in high density lipoproteins (HDL). This study was designed to determine whether an impairment in the binding of HDL to liver membrane is responsible for the hyperlipoproteinemia. Sixty male Sprague-Dawley rats were randomly divided into 2 treatments, namely copper (Cu) deficient and adequate (less than 1 and 8 mg Cu/kg of diet). After 8 weeks, plasma, heart and liver tissues were obtained. Reduction in liver Cu content and elevation in heart to body weight ratio and plasma cholesterol confirmed that rats fed the test diet were Cu-deficient. Plasma HDL isolated from both Cu-deficient and control rats were iodinated and bound to liver membranes prepared from rats of each treatment. Binding of /sup 125/I-HDL was competitively inhibited by unlabelled rat HDL from both treatments, but not by human LDL. Scatchard analysis of specific binding data showed that maximal /sup 125/I-HDL binding (per mg membrane protein) to membranes prepared from Cu-deficient rats was not lower than controls. Furthermore, the amount of /sup 125/I-HDL from deficient rats specifically bound to liver membranes prepared from either treatment was not less than the amount of /sup 125/I-HDL from control rats bound to the same membranes. The data suggest that the hyperlipoproteinemia in Cu-deficient rats may not have resulted from a decrease in the number of hepatic HDL binding sites.
- Research Organization:
- Univ. of Arizona, Tucson
- OSTI ID:
- 6846561
- Report Number(s):
- CONF-8604222-; TRN: 87-006137
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
High density lipoprotein (HDL) cholesteryl ester and protein metabolism in copper-deficient hypercholesterolemic rats
Copper deficiency and hyperlipoproteinemia induced by N,N'-bis-(2-aminoethyl)-1,3-propane diamine (2,3,2-tetramine) in rabbits
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
COPPER
NUTRITIONAL DEFICIENCY
DIGESTIVE SYSTEM DISEASES
PATHOGENESIS
LIPOPROTEINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
CELL MEMBRANES
HEART
IODINE 125
LIVER
RATS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550901 - Pathology- Tracer Techniques