Plasma zinc status and membrane lipid composition in genetically diabetic mice (db/db)
Sex and age matched diabetic C57BL/Ks-db+/db+ mice (db/db) were sacrificed at eight weeks of age. Plasma samples were collected and zinc levels determined. Livers were excised and mitochondrial and microsomal membranes prepared. Aliquots of membrane fractions were subjected to lipid extraction and cholesterol (Cl), phospholipid (PL) and fatty acid analysis (FA) performed. Plasma zinc levels in db/db mice were elevated 25% compared to m/m controls (148.8+/-8.1 ..mu..g/dl vs. 118.9+/-14.9 ..mu..g/dl). Cholesterol and PL levels remained unchanged in both mitochondrial and microsomal membranes. Analysis of PL composition from db/db mitochondria by two dimensional thin layer chromatography revealed no change in the percentage of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) but a 40% decrease in cardiolipin. Slight increases were observed in the percentage of phosphatidylserine and phosphatidylinositol (PS+PI) in microsomes isolated from db/db mice. Fatty acid analysis of microsomal PC and PE showed a decrease of 28% in the 18:1/18:0 ratio as well as a 21% decrease in the ratio of 20:4/18:2 in db/db animals. Analysis of succinate dehydrogenase (mitochondrial) and glucose-6-phosphatase (microsomal) revealed significant decreases in activity in livers of db/db mice. The altered zinc metabolism as well as the changes in membrane lipid composition suggest that this may be a model to study the role of zinc in membrane structure.
- Research Organization:
- Pennsylvania College of Podiatric Medicine, Philadelphia
- OSTI ID:
- 6846528
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
CHEMICAL COMPOSITION
DIABETES MELLITUS
PATHOGENESIS
ZINC
METABOLISM
TISSUE DISTRIBUTION
BLOOD PLASMA
CHOLESTEROL
LIPIDS
MICE
MICROSOMES
MITOCHONDRIA
OXIDOREDUCTASES
THIN-LAYER CHROMATOGRAPHY
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CELL CONSTITUENTS
CHROMATOGRAPHY
DISEASES
DISTRIBUTION
ELEMENTS
ENDOCRINE DISEASES
ENZYMES
HYDROXY COMPOUNDS
MAMMALS
MATERIALS
MEMBRANES
METABOLIC DISEASES
METALS
ORGANIC COMPOUNDS
ORGANOIDS
RODENTS
SEPARATION PROCESSES
STEROIDS
STEROLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550900 - Pathology