Control of murine macrophage H/sub 2/O/sub 2/ metabolism by amphotericin B
The authors investigated the ability of amphotericin B (AmB) to modulate H/sub 2/O/sub 2/ metabolism in murine macrophages (M theta). Following a single 0.5 mg intraperitoneal dose of AmB, AKR peritoneal M theta showed greater chemiluminescence (CL) after triggering with phorbol myristate acetate (PMA) than C57BL/6 M theta. The capacity for enhanced M theta CL was sustained for at least 2 weeks after AmB injection in AKR mice but less in C57BL/6 M theta. In other experiments, intracellular H/sub 2/O/sub 2/ metabolism was evaluated by a fluorescence activated cell sorting (FACS) assay described by Bass et al. A comparison of FACS histograms of peritoneal M theta from AmB treated and control AKR mice revealed 25-28% stimulation in the experimental group; M theta from AmB treated C57BL/6 mice showed a significant reduction of the H/sub 2/O/sub 2/ metabolism compared with resident M theta. These results are in accord with the effects of AmB on survival from experimental infection with Listeria monocytogenes. AmB enhanced survival in AKR mice while it reduced the survival of C57BL/6 mice infected with this facultative intracellular bacterium. Thus, AmB-induced resistance to infection correlates with stimulation of the M theta respiratory burst.
- Research Organization:
- Washington Univ. Medical Center, St Louis, MO
- OSTI ID:
- 6846481
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBIOTICS
BIOLOGICAL EFFECTS
HYDROGEN PEROXIDE
METABOLISM
PHORBOL ESTERS
BACTERIAL DISEASES
CHEMILUMINESCENCE
MACROPHAGES
MICE
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
CARCINOGENS
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
ESTERS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
LUMINESCENCE
MAMMALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHAGOCYTES
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology