Purification of tracer for somatomedin C radioimmunoassay by hydrophobic interaction chromatography
Researcherse have purified tracer for use in the somatomedin C radioimmunoassay by hydrophobic interaction chromatography. Material showing greatest immunoreactivity binds to Octyl Sepharose CL-4B (Pharmacia) in a buffer mixture consisting of 130 mL of acetonitrile and 870 mL of 0.1 mol/L NH4HCO3, pH 7.8, but is eluted by increasing the acetonitrile content to 180 mL/L. As compared with tracer purified by binding to specific antiserum in liquid phase, precipitating the complex with second antibody, and then dissociating by gel chromatography at acid pH, this tracer shows equal immunoreactivity against specific somatomedin C antiserum. Either preparation allows excellent discrimination between extracts of normal, acromegalic, and hypopituitary plasma samples; thus either is suitable for use in the somatomedin C radioimmunoassay. Tracer purification by hydrophobic interaction chromatography is rapid and inexpensive. It may be useful in preparing highly immunoreactive tracers for other peptide radioimmunoassays.
- Research Organization:
- Department of Endocrinology, Royal Prince Alfred Hospital, Camperdown, N.S.W., Australia
- OSTI ID:
- 6844106
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:3
- Country of Publication:
- United States
- Language:
- English
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38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
IMMUNE REACTIONS
RADIOIMMUNOASSAY
LABELLED COMPOUNDS
CHEMICAL PREPARATION
ANTIBODIES
BINDING ENERGY
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
IODINE ISOTOPES
TRACER TECHNIQUES
ENERGY
ISOTOPE APPLICATIONS
ISOTOPES
RADIOASSAY
SEPARATION PROCESSES
SYNTHESIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics
400702 - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials