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Title: Detection of ductal dysplasia in mammary outgrowths derived from carcinogen-treated virgin female BALB/c mice

Abstract

These studies were undertaken to determine if altered growth potential of mammary epithelial cells could be detected in outgrowths derived from monodispersed mammary cells of virgin female BALB/c mice previously exposed to ionizing radiation or 7, 12-dimethylbenz(a)anthracene (DMBA). Twenty-four hr prior to cell dissociation, donor animals were exposed to either 100 rads of gamma-ray irradiation, 0.25 mg of DMBA, or 0.075 mg of DMBA. Control donors were untreated. Mammary outgrowths were then derived from these donor cells by injecting either 10(5) or 10(4) cells into the gland-free mammary fat pads of three-week-old virgin female BALB/c mice. Mammary outgrowths were classified either as having a normal ductal architecture or as having ductal dysplasia. Ductal dysplasias were further classified on the basis of an index of severity, which was an arbitrary index based on the number of abnormal ductal structures within each lesion. The data indicated that treatment of donor animals with either gamma-radiation or DMBA increased the frequency of ductal lesions over control levels; however, both the frequency and severity of the lesions depended on the number of cells which were injected into the fat pad. The data indicated that ductal dysplasias were more common and more severe in outgrowths derivedmore » 10(4) rather than 10(5) cells. The ductal lesions observed in this study resembled both morphologically and histologically ductal abnormalities which have been associated with the pathogenesis of mammary carcinoma in both rats and mice.« less

Authors:
;
Publication Date:
Research Org.:
University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
OSTI Identifier:
6842823
Resource Type:
Journal Article
Journal Name:
Cancer Res.; (United States)
Additional Journal Information:
Journal Volume: 42:5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; PATHOLOGY; DIMETHYLBENZANTHRACENE; BIOLOGICAL EFFECTS; EPITHELIUM; BIOLOGICAL RADIATION EFFECTS; COMPARATIVE EVALUATIONS; GAMMA RADIATION; HISTOLOGY; MAMMARY GLANDS; MICE; NEOPLASMS; RADIOSENSITIVITY; RESEARCH PROGRAMS; ANIMAL TISSUES; ANIMALS; AROMATICS; BODY; CONDENSED AROMATICS; DISEASES; ELECTROMAGNETIC RADIATION; GLANDS; IONIZING RADIATIONS; MAMMALS; ORGANIC COMPOUNDS; ORGANS; RADIATION EFFECTS; RADIATIONS; RODENTS; TISSUES; VERTEBRATES; 550900* - Pathology; 560121 - Radiation Effects on Cells- External Source- (-1987); 550300 - Cytology

Citation Formats

Ethier, S P, and Ullrich, R L. Detection of ductal dysplasia in mammary outgrowths derived from carcinogen-treated virgin female BALB/c mice. United States: N. p., 1982. Web.
Ethier, S P, & Ullrich, R L. Detection of ductal dysplasia in mammary outgrowths derived from carcinogen-treated virgin female BALB/c mice. United States.
Ethier, S P, and Ullrich, R L. 1982. "Detection of ductal dysplasia in mammary outgrowths derived from carcinogen-treated virgin female BALB/c mice". United States.
@article{osti_6842823,
title = {Detection of ductal dysplasia in mammary outgrowths derived from carcinogen-treated virgin female BALB/c mice},
author = {Ethier, S P and Ullrich, R L},
abstractNote = {These studies were undertaken to determine if altered growth potential of mammary epithelial cells could be detected in outgrowths derived from monodispersed mammary cells of virgin female BALB/c mice previously exposed to ionizing radiation or 7, 12-dimethylbenz(a)anthracene (DMBA). Twenty-four hr prior to cell dissociation, donor animals were exposed to either 100 rads of gamma-ray irradiation, 0.25 mg of DMBA, or 0.075 mg of DMBA. Control donors were untreated. Mammary outgrowths were then derived from these donor cells by injecting either 10(5) or 10(4) cells into the gland-free mammary fat pads of three-week-old virgin female BALB/c mice. Mammary outgrowths were classified either as having a normal ductal architecture or as having ductal dysplasia. Ductal dysplasias were further classified on the basis of an index of severity, which was an arbitrary index based on the number of abnormal ductal structures within each lesion. The data indicated that treatment of donor animals with either gamma-radiation or DMBA increased the frequency of ductal lesions over control levels; however, both the frequency and severity of the lesions depended on the number of cells which were injected into the fat pad. The data indicated that ductal dysplasias were more common and more severe in outgrowths derived 10(4) rather than 10(5) cells. The ductal lesions observed in this study resembled both morphologically and histologically ductal abnormalities which have been associated with the pathogenesis of mammary carcinoma in both rats and mice.},
doi = {},
url = {https://www.osti.gov/biblio/6842823}, journal = {Cancer Res.; (United States)},
number = ,
volume = 42:5,
place = {United States},
year = {Sat May 01 00:00:00 EDT 1982},
month = {Sat May 01 00:00:00 EDT 1982}
}