31-Phosphorus nuclear magnetic resonance study of neonatal myocardial metabolism
A modified Langendorff heart preparation was designed for use with a 4.7 Tesla Nicolet NMR spectrometer operated at 80.98 MHz in the pulsed Fourier transform mode. The perfusion system delivered constant temperature (37/sup 0/C) modified Krebs-Henseleit solution containing glucose as the exogenous substrate. Experiments were performed with constant perfusion pressure, which was adjusted according to the age of the rabbit. Neonatal hearts weighted about 0.5 g. A special 16mm Dadok-type NMR probe was designed to accommodate these small hearts in a vertical bore spectrometer configuration. Spectral standardization was accomplished with a capillary tube placed in the B1 window of the probe. The principal theme of the experiments was to determine the effects of ischemic stress on the neonatal heart by measuring changes in intracellular pH, inorganic phosphate (Pi), phosphocreatine (PCr) and ATP from 31-phosphorus spectra gathered over five minute intervals by signal averaging 148 FIDs using 4K data files and 5000 Hz spectral width. Hearts were subjected to different durations of ischemic stress. The responses of neonatal hearts were compared with those of more mature hearts.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 6842241
- Report Number(s):
- CONF-8604222-; TRN: 87-008951
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ISCHEMIA
PATHOGENESIS
MYOCARDIUM
NMR SPECTRA
AGE DEPENDENCE
ATP
METABOLISM
NEONATES
NUCLEAR MAGNETIC RESONANCE
PH VALUE
PHOSPHATES
PHOSPHOCREATINE
PHOSPHORUS 31
RABBITS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
HEART
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MUSCLES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques
550600 - Medicine