Effects of prolonged moderate body deuteration on proliferative activity in major cell renewal systems in mice

Journal Article · Mon Jun 07 04:00:00 UTC 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90438-6· OSTI ID:6841693

Hodel, A ^[1]; Gebbers, J -O; Cottier, H; Laissue, J A

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1. Inst. of Pathology, Lucerne, Switzerland

To evaluate the effects of prolonged moderate body deuteration on incorporation of tritiated thymidine (/sup 3/HTdR) into the DNA of major cell renewal systems, young adult mice were given drinking fluid containing 30 % heavy water for 7, 14, 21, 42 and 70 days. Control mice drank tap water. Three hours prior to sacrifice, 925 kBq of HTdR were injected intravenously. Following extraction of the bulk of the soluble activity with an aqueous formalin solution, the residual /sup 3/H-activity of the organs was assayed by liquid scintillation counting and by autoradiography. The total thymic /sup 3/H-activity and the thymic weight, particularly of the cortex, were significantly reduced indeuterated mice early in the course of the experiment. The fraction of labeled thymocytes diminished to less than one half of the control values on day 70. The /sup 3/H-activity of the bone marrow in deuterated mice was reduced to about 3.4 of control values. In contrast, the total /sup 3/H-activity of the small intestine, as well as mean labeling index and mitotic index of small intestinal epithelia, were mot markedly altered in deuterated versus control mice. Drinking water containing 30 % of heavy water did thus not result in generalized, profound and progressive disturbance of HTdR incorporation in all the major cell renewal systems in the mouse. The thymus and, to a lesser extent, the bone marrow, were unquestionably affected, but the depression of HTdR incorporation did not increase markedly in the course of 10 weeks. In these terms, the toxicity of 30 % heavy wateer in the drinking fluid appears minor. This is of particular interest since exposure to similar concentrations is known to elicit immunodepressive and/or antineoplastic effects.

• OSTI ID: 6841693
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 30:23; ISSN LIFSA
• Country of Publication: United States
• Language: English

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560162* -- Radionuclide Effects
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
CELL PROLIFERATION
CHRONIC EXPOSURE
DIGESTIVE SYSTEM
DNA
DRINKING WATER
GASTROINTESTINAL TRACT
HEAVY WATER
HEMATOPOIETIC SYSTEM
HYDROGEN COMPOUNDS
INTESTINES
LABELLED COMPOUNDS
LIQUID SCINTILLATION DETECTORS
LYMPHATIC SYSTEM
MAMMALS
MEASURING INSTRUMENTS
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RADIATION DETECTORS
RADIATION EFFECTS
RODENTS
SCINTILLATION COUNTERS
SMALL INTESTINE
SOMATIC CELLS
THYMOCYTES
THYMUS
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER