Human cytomegalovirus replicates in gamma-irradiated fibroblasts
Because of the unique interdependence of human cytomegalovirus (HCMV) and the physiological state of the host cell, we evaluated the ability of human foreskin fibroblasts (HFF), exposed to gamma radiation, to support HCMV growth. Irradiation of HFF with 2,500 rADS prevented cellular proliferation and suppressed cellular DNA, but not RNA or protein synthesis. Treatment of HFF cells with 2,500 rADS 6 or 48 hours prior to infection did not alter the time course or virus yield during HCMV replication. Virus plaquing efficiency in irradiated cells was comparable to that of nonirradiated cells. As judged by thymidine incorporation and BUdR inhibition of virus replication, HCMV infection induced both thymidine kinase activity and host cell DNA synthesis in irradiated cells. In addition, virus could be recovered from HFF exposed to radiation 0-2 days after infection with HCMV. These studies indicate that the damage to cells by gamma irradiation does not alter the capacity of host cells to support HCMV replication.
- Research Organization:
- Veterans Administration Medical Center, Newington, CT
- OSTI ID:
- 6841464
- Journal Information:
- J. Med. Virol.; (United States), Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
RADIATION DOSES
VIRUSES
CELL PROLIFERATION
DNA REPLICATION
CELL CULTURES
GAMMA RADIATION
INHIBITION
MAN
ANIMAL CELLS
ANIMALS
CONNECTIVE TISSUE CELLS
DOSES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
MICROORGANISMS
NUCLEIC ACID REPLICATION
PARASITES
PRIMATES
RADIATIONS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture