Mechanism of ionophoric transport of indium-111 cations through a lipid bilayer membrane
The use of mobile ionophores to facilitate the transport of /sup 111/In through a lipid bilayer membrane has broad applications in liposome technology and cell labeling. However, the mechanism of such ionophore-mediated transport of /sup 111/In through a lipid bilayer membrane is not completely clear. The present report describes the correlations of the behaviors of ionophoric loading of /sup 111/In into liposomes with the lipophilicity and the indium-binding affinity of three ionophores, namely, 8-hydroxyquinoline, acetylacetone, and tropolone. Our results suggest that the mechanism of the ionophoric transport of /sup 111/In through a lipid bilayer membrane involves the rapid exchange of /sup 111/In cations among the ionophores in both the aqueous solution and the lipid bilayer. Furthermore, the effectiveness of an ionophore in facilitating the transport of /sup 111/In from the external aqueous compartment to the entrapped nitrilotriacetic acid depends not only on the lipophilicity of the (/sup 111/In)ionophore complex, but also on the lipophilicity of the free ionophore itself and the competition of /sup 111/In between nitrilotriacetic acid inside the inner aqueous compartment of the liposome and the ionophore imbedded in the lipid bilayer membrane of the liposome.
- Research Organization:
- Univ. of Southern California, Los Angeles
- OSTI ID:
- 6840506
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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