Electrophoretic analysis of myofibrillar and mitochondrial proteins from ventricular myocardium from normal and copper deficient rats
- Univ. of Wyoming, Laramie (USA)
Male Long-Evans rats were fed copper adequate (8 mg Cu/kg diet, N=9) or copper deficient (0.4 mg Cu/kg diet, N=10) diets from weanling until sacrificed (9 weeks). Animals fed the copper deficient diet had decreased hematocrit and liver copper levels and increased heart weight verifying copper deficiency. Left ventricle cardiac tissue was evaluated for changes in myofibrillar and mitochondrial proteins by gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and densitometry. No differences in individual myofibrillar proteins as a function of copper deficiency were apparent. In the mitochondrial protein fraction 2 peptides (approx. molecular weights 22,000 and 19,000) present in normal ventricles were absent from copper deficient, severely hypertrophic tissues of present in lesser amounts in copper deficient animals exhibiting a lesser degree of hypertrophy. The molecular weight of these peptides corresponds closely to published weights of cytochrome C oxidase subunits. The changes observed here may represent alterations in translation or assembly of cytochrome C oxidase as a function of copper deficiency.
- OSTI ID:
- 6832880
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Vol. 3:3; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
COPPER
BIOLOGICAL EFFECTS
MYOCARDIUM
PROTEIN STRUCTURE
ANIMAL TISSUES
CYTOCHROME OXIDASE
DIET
ELECTROPHORESIS
HEART
HYPERTROPHY
NUTRITIONAL DEFICIENCY
RATS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
ELEMENTS
ENZYMES
HAEM DEHYDROGENASES
MAMMALS
METALS
MUSCLES
ORGANS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
RODENTS
TISSUES
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology