Insulin receptor in Drosophila melanogaster
Abstract
A specific, high affinity insulin receptor is present in both adult Drosophila and in Drosophila embryos. Wheat germ lectin-enriched extracts of detergent-solubilized membranes from embryos and adults bind insulin with a K/sub d/ of 15 nM. Binding is specific for insulin; micromolar concentrations of proinsulin, IGFI, and IGFII are required to displace bound /sup 125/I-insulin. Insulin-dependent protein tyrosine kinase activity appears during embryogenesis. It is evident between 6 and 12 hours of development, peaks between 12 and 18 hours and falls in the adult. During 0-6 hours of embryogenesis, and in the adult, a specific protein band (Mr = 135,000) is crosslinked to /sup 125/I-insulin. During 6-12 and 12-18 hours of embryogenesis stages in which insulin-dependent protein tyrosine kinase is high, an additional band (Mr = 100,000) becomes crosslinked to /sup 125/I-insulin. Isolation and DNA sequence analysis of genomic clones encoding the Drosophila insulin receptor will be presented as will the characterization of insulin receptor mRNA's during development.
- Authors:
- Publication Date:
- Research Org.:
- Memorial Sloan-Kettering Cancer Center, New York, NY
- OSTI Identifier:
- 6832530
- Report Number(s):
- CONF-8606151-
Journal ID: CODEN: FEPRA; TRN: 87-006983
- Resource Type:
- Conference
- Journal Name:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
- Additional Journal Information:
- Journal Volume: 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DROSOPHILA; BIOCHEMISTRY; INSULIN; BIOCHEMICAL REACTION KINETICS; RECEPTORS; PHOSPHOTRANSFERASES; ENZYME ACTIVITY; PROTEINS; CROSS-LINKING; AFFINITY; DNA SEQUENCING; IODINE 125; MESSENGER-RNA; ONTOGENESIS; TRACER TECHNIQUES; ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; CHEMICAL REACTIONS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DIPTERA; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; FLIES; FRUIT FLIES; HORMONES; INSECTS; INTERMEDIATE MASS NUCLEI; INVERTEBRATES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MEMBRANE PROTEINS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERIZATION; RADIOISOTOPES; REACTION KINETICS; RNA; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES; 550201* - Biochemistry- Tracer Techniques
Citation Formats
Petruzzelli, L, Herrera, R, and Rosen, O. Insulin receptor in Drosophila melanogaster. United States: N. p., 1986.
Web.
Petruzzelli, L, Herrera, R, & Rosen, O. Insulin receptor in Drosophila melanogaster. United States.
Petruzzelli, L, Herrera, R, and Rosen, O. 1986.
"Insulin receptor in Drosophila melanogaster". United States.
@article{osti_6832530,
title = {Insulin receptor in Drosophila melanogaster},
author = {Petruzzelli, L and Herrera, R and Rosen, O},
abstractNote = {A specific, high affinity insulin receptor is present in both adult Drosophila and in Drosophila embryos. Wheat germ lectin-enriched extracts of detergent-solubilized membranes from embryos and adults bind insulin with a K/sub d/ of 15 nM. Binding is specific for insulin; micromolar concentrations of proinsulin, IGFI, and IGFII are required to displace bound /sup 125/I-insulin. Insulin-dependent protein tyrosine kinase activity appears during embryogenesis. It is evident between 6 and 12 hours of development, peaks between 12 and 18 hours and falls in the adult. During 0-6 hours of embryogenesis, and in the adult, a specific protein band (Mr = 135,000) is crosslinked to /sup 125/I-insulin. During 6-12 and 12-18 hours of embryogenesis stages in which insulin-dependent protein tyrosine kinase is high, an additional band (Mr = 100,000) becomes crosslinked to /sup 125/I-insulin. Isolation and DNA sequence analysis of genomic clones encoding the Drosophila insulin receptor will be presented as will the characterization of insulin receptor mRNA's during development.},
doi = {},
url = {https://www.osti.gov/biblio/6832530},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:6,
place = {United States},
year = {Thu May 01 00:00:00 EDT 1986},
month = {Thu May 01 00:00:00 EDT 1986}
}