Stimulation of phosphoinositide hydrolysis by a novel substance partially purified from rat and bovine brain
This study demonstrates the partial purification of a potentially novel substance from rat and bovine brain. Whole brains were homogenized in distilled water, then heated at 100/sup 0/C for 30 min. The water extract was dialyzed and the < 2000 dalton dialysate was fractionated on a Bio-Gel P-4 gel filtration column. Fractions were assayed for stimulation of /sup 3/H-inositol monophosphate (/sup 3/H-IP) using lithium-treated slices of rat cerebral cortex prelabelled with /sup 3/H-myo-inositol. A major peak of activity was observed in fractions from the molecular weight range of 800-1300 daltons. Stimulation of phosphoinositide hydrolysis by this material was time-dependent and dose-related. Maximal stimulation of /sup 3/H-IP (323% of control) required 10mg/ml of bovine material and was observed at 30 minutes. These effects could not be mimicked by a number of substances of similar molecular weight (e.g. substance P, neurotensin, angiotensin II, bradykinin). Furthermore, the effects of this material were not blocked by antagonist drugs which act at the alpha-adrenoceptor, muscarinic cholinoceptor, 5-HT2 receptor, substance P receptor, or neurotensin receptor. These results indicate that the substance isolated may be a novel neuroactive molecule which has receptors coupled to phosphoinositide hydrolysis in brain.
- Research Organization:
- Marshall Univ. School of Medicine, Huntington, WV
- OSTI ID:
- 6831933
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of the phosphoinositide pathway in cultured Sertoli cells from immature rats: effects of follicle-stimulating hormone and fluoride
Role of calcium in phosphoinositide metabolism and inhibition of norepinephrine transport into synaptic vesicles by amphetamine analogs
Related Subjects
PHOSPHOLIPIDS
HYDROLYSIS
PROTEINS
PURIFICATION
BIOCHEMICAL REACTION KINETICS
BRAIN
CATTLE
DOSE-RESPONSE RELATIONSHIPS
INOSITOL
MOLECULAR WEIGHT
RATS
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DECOMPOSITION
DOMESTIC ANIMALS
ESTERS
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
LYSIS
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
REACTION KINETICS
RODENTS
RUMINANTS
SACCHARIDES
SOLVOLYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques