Image analysis of cells stained by fluorescent in situ hybridization
- Leiden Univ. (Netherlands)
Specific nucleic acid sequences of about 1 kb can be detected on a routine basis using FISH methods. This is achieved by indirect techniques and conventional microscopy, or with fluorescent probes (direct methods) and integrating detection devices (e.g. CCD cameras). Labeling of probes with defined ratios of two or three fluorophores allows localization of multiple targets, especially when the signals are quantified by image analysis. This requires fluoroescence microscopes equipped with multi band pass filters and computer controlled filter wheels for sequential recording of red, green and blue images using a B/W CCD camera, and correction of occurring image shifts. This combined approach was applied to localize and map genes on chromosomes. In case of nuclei with long extentions of almost linear DNA molecules, (so-called halo preparations), single hybridization sides could be shown.
- OSTI ID:
- 6831786
- Report Number(s):
- CONF-9303114-; CODEN: CYTODQ
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 6; Conference: 16. congress of the International Society for Analytical Cytology, Colorado Springs, CO (United States), 21-26 Mar 1993; ISSN 0196-4763
- Country of Publication:
- United States
- Language:
- English
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