Features of target cell lysis by class I and class II MHC restricted cytolytic T lymphocytes

Journal Article · Mon Dec 01 00:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:6831750

Maimone, M M; Morrison, L A; Braciale, V L; Braciale, T J

The lytic activity of influenza virus-specific muvine cytolytic T lymphocyte (CTL) clones that are restricted by either H-2K/D (class I) or H-2I (class II) major histocompatibility (MHC) locus products was compared on an influenza virus-infected target cell expressing both K/D and I locus products. With the use of two in vitro measurements of cytotoxicity, conventional /sup 51/Cr release, and detergent-releasable radiolabeled DNA (as a measure of nuclear disintegration in the early post-lethal hit period), the authors found no difference between class I and class II MHC-restricted CTL in the kinetics of target cell destruction. In addition, class II MHC-restricted antiviral CTL failed to show any lysis of radiolabeled bystander cells. Killing of labeled specific targets by these class II MHC-restricted CTL was also efficiently inhibited by unlabeled specific competitor cells in a cold target inhibition assay. In sum, these data suggest that class I and class II MHC-restricted CTL mediate target cell destruction by an essentially similar direct mechanism.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 6831750
• Journal Information: J. Immunol.; (United States), Vol. 137:11
• Country of Publication: United States
• Language: English

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