Localization of Legionella pneumophila in tissue using FITC-conjugated specific antibody and a background stain

Journal Article · Sat May 01 00:00:00 EDT 1982 · Am. J. Clin. Pathol.; (United States)

DOI:https://doi.org/10.1093/ajcp/77.5.601· OSTI ID:6816307

Lowry, B S; Vega, Jr, F G; Hedlund, K W

Lightly staining formalin-fixed or fresh tissue with Gram's crystal violet obviates interfering nonspecific fluorescence by acting as a metachromatic stain in ultraviolet light. Against the easily recognized background of tissues and cells fluorescein isothiocyanate-tagged Legionella pneumophila antibodies can then identify this bacterium in or on individual cells. This procedure can be run at room temperature in two hours and has the potential for further widespread applicability.

• Research Organization: U.S. Army Medical Research Institute of Infectious Disease, Fort Detrick, Frederick, Maryland
• OSTI ID: 6816307
• Journal Information: Am. J. Clin. Pathol.; (United States), Vol. 77:5
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
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