Action of cholinergic poisons on the central nervous system and effectiveness of potential antidotes. Annual report 1 Jul 81-30 Jun 82
The research aim was to determine the effects of soman, related organophosphate toxins and potential antidotes on brain regional functions in rats: The (/sup 14/C)-2-deoxyglucose procedure (2-DG) was used for mapping brain regional glucose use. Quantitative autoradiography was used for muscarinic and nicotinic cholinergic receptors. The 2-DG procedure gives a quantitative measure of glucose utilization in brain regions and is in index of the 'functional activity' in brain regions and systems. Values were determined in controls, rats with soman induced seizures, seizures induced by convulsants (DFP, strychnine, picrotoxin, pentylenetetrazol, penicillin) and soman pretreated with TAB. Brain regional cholinergic receptor maps were prepared and some regional muscarinic and nicotinic receptor densities have been quantified. Soman (112 micrograms/kg i.m.) causes strong, continuous seizures and a dramatic (2-6 fold) increase in the rate of glucose use in 10 major brain regions. Most intense increases were in septum, substants nigra reticularis and outer layer of hippcampal dendata gyrus. The overt seizures of rats induced by convulsants DFP, strychnine, picrotoxin, pentylenetetrazol and penicillin (in hippocampus) were strikingly different from that of rats with soman seizures. High doses (2X LD50) of soman in rats protected with TAB caused a 50% depression of glucose use in most brain regions. The effects of repeated soman exposure on muscarinic and nicotinic receptors are under study.
- Research Organization:
- Kansas Univ., Kansas City (USA). Medical Center
- OSTI ID:
- 6815368
- Report Number(s):
- AD-A-137900/7
- Country of Publication:
- United States
- Language:
- English
Similar Records
Role of glutamatergic system in nerve agent intoxication
Dizocilpine (MK-801) arrests status epilepticus and prevents brain damage induced by Soman. (Reannouncement with new availability information)
Related Subjects
BRAIN
METABOLISM
ORGANIC PHOSPHORUS COMPOUNDS
TISSUE DISTRIBUTION
TOXICITY
ACETYLCHOLINE
ANTIMETABOLITES
AUTORADIOGRAPHY
CENTRAL NERVOUS SYSTEM
CHEMORECEPTORS
GLUCOSE
HIPPOCAMPUS
RATS
ALDEHYDES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOHYDRATES
DISTRIBUTION
DRUGS
ESTERS
HEXOSES
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RODENTS
SACCHARIDES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)