Oxygen isotope ratio measurements on carbon dioxide generated by reaction of microliter quantities of biological fluids with guanidine hydrochloride
Guanidine hydrochloride was used to convert water in biological fluids to carbon dioxide for oxygen isotope ratio measurements. Five 10-..mu..L aliquots each of five different saliva, urine, plasma, and human milk samples were allowed to react with 100 mg of guanidine hydrochloride at 260/sup 0/C to produce ammonia and carbon dioxide. Ammonia was removed with 100% phosphoric acid and carbon dioxide was cryogenically purified before isotope ratio measurement. At natural abundances, the delta/sup 18/O values of the biological fluids were reproducible to within 0.16% (standard deviation) and accurate to within 0.11 +/- 0.73% (x vector +/- SD) of the H/sub 2/O-CO/sub 2/ equilibration values. At a 250% enrichment level of /sup 18/O, the delta/sup 18/O values of the biological fluids were reproducible to within 0.95% and accurate to -1.27 +/- 2.25%.
- Research Organization:
- Baylor College of Medicine, Houston, TX
- OSTI ID:
- 6813682
- Journal Information:
- Anal. Chem.; (United States), Vol. 59:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BLOOD PLASMA
CHEMICAL REACTIONS
CARBON DIOXIDE
MASS SPECTROSCOPY
GUANIDINES
MILK
OXYGEN 16
ISOTOPE RATIO
OXYGEN 18
SALIVA
URINE
WATER
REMOVAL
ABUNDANCE
AMMONIA
EXPERIMENTAL DATA
HYDROCHLORIC ACID
NATURAL OCCURRENCE
QUANTITATIVE CHEMICAL ANALYSIS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
CARBON COMPOUNDS
CARBON OXIDES
CARBONIC ACID DERIVATIVES
CHALCOGENIDES
CHEMICAL ANALYSIS
DATA
EVEN-EVEN NUCLEI
FOOD
HYDRIDES
HYDROGEN COMPOUNDS
INFORMATION
INORGANIC ACIDS
ISOTOPES
LIGHT NUCLEI
MATERIALS
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NUCLEI
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
OXYGEN ISOTOPES
SPECTROSCOPY
STABLE ISOTOPES
WASTES
400105* - Separation Procedures
400102 - Chemical & Spectral Procedures