Mechanism and applicability of hydrolysis of peptides and proteins utilizing Pt(II) complexes
The hydrolysis of amino acid esters and amides has been achieved by using organic reagents, strongly acidic and basic solutions, and transition metal complexes. However, enzymes have always been able to surpass these methods in terms of speed of the hydrolysis and the mild conditions necessary to observe hydrolysis. In order to understand how enzymes undergo their reactions with such remarkable speed and efficiency, researchers are studying and developing inorganic reagents which can facilitate the hydrolysis of peptide bonds. The treatment of the tripetide {gamma}-glutamyl-cyteinylglycine with one equivalent of PtCl{sub 4}{sup {minus}2} results in hydrolysis of the cysteinyl-glycine bond. The reaction is strongly dependent on the amount of chloride ion in solution and also shows a lesser dependence on ionic strength and pH. Hydrolysis is promoted through a chelate interaction of the platinum with the sulfur of cysteine and the carbonyl oxygen of the amide bond. The hydrolysis of proteins was then undertaken. Yeast cytochrome c and the subunits of hemoglobin were examined to determine if PtCl{sub 4}{sup {minus}2} or Pt(en)Cl{sub 2} could promote cleavage of the peptide bond next to a cysteine residue. It appears that hydrolysis of the peptide bond to the right of the cysteinly side chain has been realized. 51 refs., 12 figs., 12 tabs.
- Research Organization:
- Ames Lab., Ames, IA (United States)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6812379
- Report Number(s):
- IS-T-1468; ON: DE90012448; TRN: 90-021738
- Resource Relation:
- Other Information: Thesis (M.S.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMIDES
HYDROLYSIS
AMINO ACIDS
ESTERS
CHEMICAL REACTIONS
COMPLEXES
CYSTEINE
ENZYMES
EQUIPMENT
GLUTATHIONE
GLYCINE
HEMOGLOBIN
METALS
MODIFICATIONS
NMR SPECTRA
PEPTIDES
PLATINUM COMPLEXES
PROTEINS
REAGENTS
YEASTS
CARBOXYLIC ACIDS
DECOMPOSITION
DRUGS
ELEMENTS
EUMYCOTA
FUNGI
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LYSIS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PLANTS
POLYPEPTIDES
PORPHYRINS
RADIOPROTECTIVE SUBSTANCES
SOLVOLYSIS
SPECTRA
THIOLS
TRANSITION ELEMENT COMPLEXES
440103* - Radiation Instrumentation- Nuclear Spectroscopic Instrumentation
550200 - Biochemistry