The evolutionary conservation of DNA polymerase. alpha

Journal Article · Thu Aug 25 00:00:00 EDT 1988 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/16.16.7961· OSTI ID:6810784

Miller, M A; Korn, D; Wang, T S.F. ^[1]

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1. Stanford Univ., CA (USA)

The evolutionary conservation of DNA polymerase {alpha} was assessed by immunological and molecular genetic approaches. Four anti-human KB cell DNA polymerase {alpha} monoclonal antibodies were tested for their ability to recognize a phylogenetically broad array of eukaryotic DNA polymerases. While the single non-neutralizing antibody used in this study recognizes higher mammalian (human, simian, canine, and bovine) polymerases only, three neutralizing antibodies exhibit greater, but variable, extents of cross-reactivity among vertebrate species. Genomic Southern hybridization studies with the cDNA of the human DNA polymerase {alpha} catalytic polypeptide identify the existence of many consensus DNA sequences within the DNA polymerase genes of vertebrate, invertebrate, plant and unicellular organisms. These findings illustrate the differential evolutionary conservation of four unique epitopes on DNA sequences, presumably reflective of critical functional domains, in the DNA polymerase genes from a broad diversity of living forms.

• OSTI ID: 6810784
• Journal Information: Nucleic Acids Research; (UK), Vol. 16:16; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
BIOLOGICAL EVOLUTION
RECOMBINANT DNA
DNA HYBRIDIZATION
ANIMAL CELLS
MAN
MONOCLONAL ANTIBODIES
ANIMALS
ANTIBODIES
DNA
ENZYMES
HYBRIDIZATION
MAMMALS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
TRANSFERASES
VERTEBRATES
550200* - Biochemistry