Close correlation between restriction fragment length polymorphism of the L-MYC gene and metastasis of human lung cancer to the lymph nodes and other organs

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2353· OSTI ID:6810596

Kawashima, Kazuko; Shikama, Hiroshi; Imoto, Kazuhiko; Izawa, Mitsuo; Nishimura, Susumu ^[1]; Naruke, Tsuguo; Okabayashi, Kenzo ^[2]

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1. National Cancer Center Research Institute, Tokyo (Japan)
2. National Cancer Center Hospital, Tokyo (Japan)

Restriction length fragment polymorphism of the L-MYC gene was examined in DNAs from lung cancer tissues and normal tissues of 51 Japanese patients with lung cancer. In individual patients, no difference was seen between the restriction length fragments of the two alleles of L-MYC (6-kilobase (kb)) and 10-kb fragments in EcoRI digests in lung cancer tissues and normal tissues. But a striking correlation was found between the restriction length fragment polymorphism pattern of L-MYC and the extent of metastasis, particularly to the lymph nodes at the time of surgery: Patients with only the L band (10 kb) had few lymph node metastatic lesions, whereas patients with either the S band (6 kb) or the S and L bands almost always had lymph node metastatic lesion. A similar correlation was found between the presence of the S band and metastases to other organs. This correlation was particularly marked in cases of adenocarcinoma. These results indicate a clear genetic influence on metastases and a consequent poor prognosis for certain patients of lung cancer; L-MYC restriction length fragment polymorphism is thus shown to be a useful marker for predicting the metastatic potential of human lung cancer.

• OSTI ID: 6810596
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:7; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
AUTORADIOGRAPHY
METASTASES
GENETICS
ONCOGENES
RFLPS
ELECTROPHORESIS
HUMAN POPULATIONS
JAPAN
LUNGS
LYMPH NODES
PATIENTS
PHOSPHORUS 32
ASIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BODY
DAYS LIVING RADIOISOTOPES
GENES
ISOTOPES
LIGHT NUCLEI
LYMPHATIC SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
POPULATIONS
RADIOISOTOPES
RESPIRATORY SYSTEM
550201* - Biochemistry- Tracer Techniques